Purification of lysozyme from human neutrophils, and development of an ELISA for quantification in cells and plasma.
K Lollike, L Kjeldsen, H Sengeløv, N Borregaard - Leukemia, 1995 - europepmc.org
K Lollike, L Kjeldsen, H Sengeløv, N Borregaard
Leukemia, 1995•europepmc.orgLysozyme was purified from exocytosed granule material from PMA-stimulated human
neutrophils by polyethyleneglycol precipitation, cation exchange chromatography and
molecular sieve chromatography. Rabbit antibodies were biotinylated and affinity purified on
a lysozyme column, for subsequent development of a novel ELISA. This ELISA for lysozyme
is sensitive and accurate, and applicable to determination of lysozyme in neutrophils and
plasma.
neutrophils by polyethyleneglycol precipitation, cation exchange chromatography and
molecular sieve chromatography. Rabbit antibodies were biotinylated and affinity purified on
a lysozyme column, for subsequent development of a novel ELISA. This ELISA for lysozyme
is sensitive and accurate, and applicable to determination of lysozyme in neutrophils and
plasma.
Lysozyme was purified from exocytosed granule material from PMA-stimulated human neutrophils by polyethyleneglycol precipitation, cation exchange chromatography and molecular sieve chromatography. Rabbit antibodies were biotinylated and affinity purified on a lysozyme column, for subsequent development of a novel ELISA. This ELISA for lysozyme is sensitive and accurate, and applicable to determination of lysozyme in neutrophils and plasma.
europepmc.org